Title:
Flexural Strength Recovery Induced by Vegetative Bacteria Added to Mortar
Author(s):
Sarah L. Williams, Nazmus Sakib, Mary Jo Kirisits, and Raissa D. Ferron
Publication:
Materials Journal
Volume:
113
Issue:
4
Appears on pages(s):
523-531
Keywords:
bioinspired; biomineralization; calcium carbonate; flexural strength; self-healing; strength recovery
DOI:
10.14359/51688831
Date:
7/1/2016
Abstract:
Recent interest has focused on developing a bioinspired, selfhealing concrete in which microorganisms induce calcium carbonate precipitation to repair cracks. Although initial studies in this area have produced promising results, the ability of such microorganisms to restore structural integrity to the material through the recovery of mechanical properties (for example, strength and stiffness) has not been fully addressed. Previous studies have focused primarily on the self-sealing (that is, crack closure) of cracks as indicated by visual observation, ultrasonic test methods, or water permeability. While crack-sealing in concrete is certainly beneficial for durability, a true self-healing concrete should restore structural integrity by providing strength recovery. In the current study, the efficacy of various bacteria-based repair agents for cracked mortar beams was evaluated. Precracked mortar beams subjected to various bacterial treatments exhibited greater flexural strength recovery (8 to 30% recovery) as compared to that of precracked mortar beams without bacteria (3 to 5% recovery).
Related References:
1. Li, V., and Herbert, E., “Robust Self-Healing Concrete for Sustainable Infrastructure,” Journal of Advanced Concrete Technology, V. 10, No. 6, 2012, pp. 207-218. doi: 10.3151/jact.10.207
2. Achal, V.; Mukerjee, A.; and Reddy, M. S., “Biogenic Treatment Improves the Durability and Remediates the Cracks of Concrete Structures,” Construction & Building Materials, V. 48, 2013, pp. 1-5. doi: 10.1016/j.conbuildmat.2013.06.061
3. Liu, S.; Basaran, Z.; Zhu, J.; and Ferron, R., “Monitoring the Self-Healing Process of Biomimetic Mortar using Coda Wave Interferometry Method,” AIP Conference Proceedings 1581, Baltimore, MD, 2014, 799 pp.
4. Jonkers, H.; Thijssen, A.; Muyzer, G.; Copuroglu, O.; and Schlangen, E., “Application of Bacteria as Self-Healing Agent for the Development of Sustainable Concrete,” Ecological Engineering, V. 36, No. 2, 2010, pp. 230-235. doi: 10.1016/j.ecoleng.2008.12.036
5. Ramachandran, S.; Ramakrishnan, V.; and Bang, S., “Remediation of Concrete Using Micro-Organisms,” ACI Materials Journal, V. 98, No. 1, Jan.-Feb. 2001, pp. 3-9.
6. Van Tittelboom, K.; De Belie, N.; De Muynck, W.; and Verstraete, W., “Use of Bacteria to Repair Cracks in Concrete,” Cement and Concrete Research, V. 40, No. 1, 2010, pp. 157-166. doi: 10.1016/j.cemconres.2009.08.025
7. Wang, J.; Soens, H.; Verstraete, W.; and De Belie, N., “Self-Healing Concrete by Use of Microencapsulated Bacterial Spores,” Cement and Concrete Research, V. 56, 2014, pp. 139-152. doi: 10.1016/j.cemconres.2013.11.009
8. Basaran, Z., “Biomineralization of Cement Based Materials: Inoculation of Vegetative Cells,” The University of Texas at Austin, Austin, TX, 2013.
9. Achal, V.; Mukherjee, A.; and Reddy, M., “Microbial Concrete: Way to Enhance the Durability of Structures,” Journal of Materials in Civil Engineering, V. 23, No. 6, 2011, pp. 730-734. doi: 10.1061/(ASCE)MT.1943-5533.0000159
10. Achal, V.; Mukherjee, A.; and Reddy, M., “Effect of Calcifying Bacteria on Permeation Properties of Concrete,” Journal of Industrial Microbiology & Biotechnology, V. 38, No. 9, 2011, pp. 1229-1234. doi: 10.1007/s10295-010-0901-8
11. De Muynck, W.; Debrouwer, D.; De Belie, N.; and Verstraete, W., “Bacterial Carbonate Precipitation Improves the Durability of Cementitious Materials,” Cement and Concrete Research, V. 38, No. 7, 2008, pp. 1005-1014. doi: 10.1016/j.cemconres.2008.03.005
12. Zhang, B.; Bundur, Z.; Mondal, P.; and Ferron, R., “Use of Biomineralization in Developing Smart Concrete Inspired by Nature,” International Journal of Materials and Structural Integrity, V. 9, No. 1/2/3, 2015, pp. 39-60. doi: 10.1504/IJMSI.2015.071109
13. Bang, S.; Lippert, J.; Yerra, U.; Mulukutla, S.; and Ramakrishnan, V., “Microbial Calcite, a Bio-Based Smart Nanomaterial in Concrete Remediation,” International Journal of Smart and Nano Materials, V. 1, No. 1, 2010, pp. 28-39. doi: 10.1080/19475411003593451
14. Patil, H.; Prashant, H.; Raijiwala, D.; and Vijay, B., “Bacterial Concrete—A Self-Healing Concrete,” International Journal of Applied Engineering Research, V. 3, No. 12, 2008, pp. 1719-1725.
15. Buchanan, R.; Gibbons, N.; Cowan, S.; Holt, J.; Liston, J.; Murray, R.; Niven, C.; Ravin, A.; and Stanier, R., Bergey’s Manual of Determinative Bacteriology, eighth edition, The Williams & Wilkin Company, Baltimore, MD, 1974.
16. Bang, S.; Galinat, J.; and Ramakrishnan, V., “Calcite Precipitation Induced by Polyurethane-Immobilized Bacillus pasteurii,” Enzyme and Microbial Technology, V. 28, No. 4-5, 2001, pp. 404-409. doi: 10.1016/S0141-0229(00)00348-3
17. Wang, J.; De Belie, N.; and Verstraete, W., “Diatomaceous Earth as a Protective Vehicle for Bacteria Applied for Self-Healing Concrete,” Journal of Industrial Microbiology & Biotechnology, V. 39, No. 4, 2012, pp. 567-577. doi: 10.1007/s10295-011-1037-1
18. Wiktor, V., and Jonkers, H., “Quantification of Crack-Healing in Novel Bacteria-Based Self-Healing Concrete,” Cement and Concrete Composites, V. 33, No. 7, 2011, pp. 763-770. doi: 10.1016/j.cemconcomp.2011.03.012
19. Silva, F.; Boon, N.; De Belie, N.; and Verstraete, W., “Industrial Application of Biological Self-Healing Concrete: Challenges and Economic Feasibility,” Journal of Commercial Biotechnology, V. 21, No. 1, 2015, pp. 31-38. doi: 10.5912/jcb662
20. ASTM C150/C150M-15, “Standard Specification for Portland Cement,” ASTM International, West Conshohocken, 2015, 9 pp.
21. ASTM C33/C33M-13, “Standard Specification for Concrete Aggregates,” ASTM International, West Conshohocken, 2013, 11 pp.
22. Williams, S.; Kirisits, M.; and Ferron, R., “Optimization of Growth Medium for Sporosarcina pasteurii in Bio-Based Cement Pastes to Mitigate Delay in Hydration Kinetics,” Journal of Industrial Microbiology & Biotechnology, V. 43, No. 4, 2016, pp. 567-575. doi: 10.1007/s10295-015-1726-2
23. Williams, S., “Toward the Development of Robust Self-Healing Concrete Using Vegetative Microorganisms,” master’s thesis, The University of Texas at Austin, Austin, TX, 2015.
24. ASTM C305-14, “Standard Practice for Mechanical Mixing of Hydraulic Cement Pastes and Mortars of Plastic Consistency,” ASTM International, West Conshohocken, PA, 2014, 3 pp.
25. Sani, F., and Todman, J., Experimental Design and Statistics for Psychology: A First Course, first edition, Wiley, Hoboken, NJ, 2008, 234 pp.
26. Bundur, Z.; Kirisits, M. J.; and Ferron, R. D., “Biomineralized Cement-Based Materials: Impact of Inoculating Vegetative Bacterial Cells on Hydration and Strength,” Cement and Concrete Research, V. 67, 2015, pp. 237-245. doi: 10.1016/j.cemconres.2014.10.002
27. Park, S.; Park, Y.; Chun, W.; Kim, W.; and Ghim, S., “Calcite-Forming Bacteria for Compressive Strength Improvement in Mortar,” Journal of Microbiology and Biotechnology, V. 20, No. 4, 2010, pp. 782-788.
28. Ghosh, P.; Mandal, S.; Chattopadhyay, B.; and Pal, S., “Use of Microorganism to Improve the Strength of Cement Mortar,” Cement and Concrete Research, V. 35, No. 10, 2005, pp. 1980-1983. doi: 10.1016/j.cemconres.2005.03.005
29. Edvardsen, C., “Water Permeability and Autogenous Healing of Cracks in Concrete,” ACI Materials Journal, V. 96, No. 4, July-Aug. 1999, pp. 448-455.
30. Reinhardt, H., and Jooss, M., “Permeability and Self-Healing of Cracked Concrete as a Function of Temperature and Crack Width,” Cement and Concrete Research, V. 33, No. 7, 2003, pp. 981-985. doi: 10.1016/S0008-8846(02)01099-2
31. Wu, M.; Johannesson, B.; and Geiker, M., “A Review: Self-Healing in Cementitious Materials and Engineered Cementitious Composite as a Self-Healing Material,” Construction & Building Materials, V. 28, No. 1, 2012, pp. 571-583. doi: 10.1016/j.conbuildmat.2011.08.086
32. Van Tittelboom, K., and De Belie, N., “Self-Healing in Cementitious Materials—A Review,” Materials (Basel), V. 6, No. 6, 2013, pp. 2182-2217. doi: 10.3390/ma6062182